To analyze the number of satellite cells and fibroadipose progenitor cells (FAPs) in injured muscles, muscles were dissected and digested with dispase II and collagenase II as previously described.[53 (link)
] Isolated mononucleated cells were immunostained with the following antibodies: 1:400 CD45‐PE (clone 30‐F11; BD Biosciences), 1:4000 Sca‐1‐PE‐Cy7 (clone D7, BD Biosciences), 1:400 CD31‐PE (clone 390; eBiosciences). Fibroadipose progenitor cells were counted using the following criteria: CD31−/CD45−/Sca1+ and satellite cells are counted by tdTomato+ using BD LSR II cytometry analyzer and analyzed using FCS Expression 6 Flow software 6.01.
] Isolated mononucleated cells were immunostained with the following antibodies: 1:400 CD45‐PE (clone 30‐F11; BD Biosciences), 1:4000 Sca‐1‐PE‐Cy7 (clone D7, BD Biosciences), 1:400 CD31‐PE (clone 390; eBiosciences). Fibroadipose progenitor cells were counted using the following criteria: CD31−/CD45−/Sca1+ and satellite cells are counted by tdTomato+ using BD LSR II cytometry analyzer and analyzed using FCS Expression 6 Flow software 6.01.
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