OCT (Envisu R4310, Leica, Wetzlar, Germany) was conducted as previously described [22 (link),27 (link)]. Briefly, mice were subjected to mydriasis by a combination of 0.5% tropicamide and 0.5% phenylephrine (Santen Pharmaceutical, Osaka, Japan). After 5 min, mice were anesthetized as same as Section 4.2 . Anesthetized mice were quickly subjected to OCT analyses. B-scan images were obtained from equatorial slices of en-face scans, and images in 0.2, 0.4, and 0.6 mm from the optic nerve head were taken. Retinal thickness was measured from the outer retina to the inner retina as we described [27 (link)].
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