ATAC-seq was performed using the Nextera Tn5 transposase (Illumina) on 6 × 104 THP1 cells and whole BM from an MLL-AF4 ALL pediatric patient, as previously described [18 (link), 39 (link)]. Libraries were sequenced by paired-end sequencing using a 75 cycle high output kit on a Nextseq 500 (Illumina).
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