EGFP-p27highmCherry-Ki67low quiescent (Q) and cycling (C) cells seeded in 96-well plates (5 × 103 cells per well) were subjected to DNA synthesis assays using the BrdU Assay kit (Cell Signalling) as described previously 22 (link). Absorbance was read at 450 nm using a Synergy™ 2 multidetection microplate reader (BioTek, VT).
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