The concentrations of IgA in GW, SMGs, and in CC and fecal homogenates were quantified by a sandwich ELISA (Bethyl Laboratories Inc., Montgomery, AL, USA) as previously described [49 (link)]. The IgA content in SMGs and CC was normalized by total protein concentration which was measured using the Pierce® 660 nm Protein Assay Reagent (Thermo Fisher Scientific) following the manufacturer’s instructions.
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