Immunohistochemical Analysis of ACE2 and TMPRSS2 in NAFLD

Immunohistochemistry was performed on biopsies obtained from 6 NAFLD patients for which formalin-fixed liver sections were available, using the rabbit anti-human ACE2 (ab108252) and rabbit anti-human TMPRSS2 (ab109131, Abcam, Cambridge, UK), both diluted at 1:6400 according to the manufacturer’s instructions, and developed using DAB (3,3′-Diaminobenzidine, Vector Labs) as chromogen as previously described [22 (link)]. Histological images were taken using the BX41 microscope (Olympus), coupled with Leica LAS X Life Science software.

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Rosso C., Demelas C., Agostini G., Abate M.L., Vernero M., Caviglia G.P., D’Amato D., Armandi A., Tapparo M., Guariglia M., Troshina G., Massano A., Olivero A., Nicolosi A., Zannetti A., Pellicano R., Ciancio A., Saracco G.M., Ribaldone D.G., Bugianesi E, & Fagoonee S. (2022). Expression of SARS-Cov-2 Entry Factors in Patients with Chronic Hepatitis. Viruses, 14(11), 2397.

Publication 2022

ab108252 ab109131 DAB BX41 microscope Leica LAS X Life Science software

Ace2 Biopsies Chromogen Formalin Human Immunohistochemistry Liver Microscope Nafld Patients Rabbit Tmprss2 Vector

Corresponding Organization : Institute of Biostructure and Bioimaging

Other organizations : Azienda Ospedaliera Citta' della Salute e della Scienza di Torino

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Variable analysis

independent variables

Anti-human ACE2 antibody (ab108252)
Anti-human TMPRSS2 antibody (ab109131)

dependent variables

ACE2 and TMPRSS2 protein expression levels in liver sections

control variables

Formalin-fixed liver sections from 6 NAFLD patients
Staining with DAB (3,3′-Diaminobenzidine) as chromogen

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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