Characterization of ccRCC Cell Lines

ACHN, A498, Caki1, and Caki2 ccRCC cell lines were purchased from American Type Culture Collection (Manassas, VA). KIJ265T and RWV366T were developed in the Copland Laboratory as previously described [24 (link), 58 (link)]. 786-O and UMRC3 were a kind gift from Dr. B. H. Grossman [59 (link)]. VHL mutational and deletional status were examined via DNA sequencing and multiplex ligation dependent probe amplification, respectively. Cells were maintained in DMEM (Cellgro) supplemented with 5% FBS (Hyclone) and 1% penicillin-streptomycin-amphotericin B (Cellgro) at 37°C with 5% CO₂.

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von Roemeling C.A., Marlow L.A., Radisky D.C., Rohl A., Larsen H.E., Wei J., Sasinowska H., Zhu H., Drake R., Sasinowski M., Tun H.W, & Copland J.A. (2014). Functional genomics identifies novel genes essential for clear cell renal cell carcinoma tumor cell proliferation and migration. Oncotarget, 5(14), 5320-5334.

Publication 2014

Caki1 DMEM penicillin-streptomycin-amphotericin B

Amphotericin Amphotericin b Cell lines Cells Deletional Multiplex ligation dependent probe amplification Mutational Penicillin Penicillin b Streptomycin

Corresponding Organization : Mayo Clinic in Florida

Other organizations : Incogen (United States)

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Variable analysis

independent variables

Cell lines: ACHN, A498, Caki1, Caki2, KIJ265T, RWV366T, 786-O, UMRC3

dependent variables

VHL mutational and deletional status

control variables

Cell culture conditions: DMEM supplemented with 5% FBS and 1% penicillin-streptomycin-amphotericin B, maintained at 37°C with 5% CO2

controls

Positive control: None specified
Negative control: None specified

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