Fungal Colony Morphology and Growth Assay

Colony morphology and growth rate were assayed with potato dextrose agar (PDA) cultures grown at 25°C for three days. Conidiation was assayed with 5-day-old CMC cultures as described [20] (link), [70] (link). Conidium morphology was examined and photographed with an Olympus BX-51 microscope. For assaying conidium germination and germ tube growth, freshly harvested conidia were cultured in liquid YEPD medium for 12 h. Slab cultures grown on a thin layer of complete medium (CM) for 36 h were examined for defects in hyphal tip growth and branching [20] (link), [70] (link).

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Wang C., Zhang S., Hou R., Zhao Z., Zheng Q., Xu Q., Zheng D., Wang G., Liu H., Gao X., Ma J.W., Kistler H.C., Kang Z, & Xu J.R. (2011). Functional Analysis of the Kinome of the Wheat Scab Fungus Fusarium graminearum. PLoS Pathogens, 7(12), e1002460.

Publication 2011

Agar Conidia Dextrose Germination Hyphal Microscope Potato

Corresponding Organization :

Other organizations : Northwest A&F University, Purdue University West Lafayette, Cereal Disease Laboratory, University of Minnesota

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Variable analysis

independent variables

Culture growth temperature (25°C)
Culture growth duration (3 days for PDA, 5 days for CMC)
Culture medium (PDA, CMC, YEPD, CM)

dependent variables

Colony morphology
Growth rate
Conidiation
Conidium morphology
Conidium germination
Germ tube growth
Hyphal tip growth
Branching

control variables

Not explicitly mentioned

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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