Comprehensive Liver Histology and Function Assessment

Liver histology was evaluated by standard H&E staining in paraffin-embedded liver sections (4–6 μm). Following H&E staining, images were captured by Olympus Image Pro-Analyzer software 7.0 (Olympus, Tokyo, Japan) and evaluated by a board-certified pathologist. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALK PHOS), and total bilirubin were measured by a Dimension RxL Max Integrated Chemistry system (Dade Behring Inc., Deerfield IL) at the Department of Chemistry, Baylor Scott & White Healthcare, Temple TX. Biliary senescence was evaluated in frozen liver sections (10 μm) by staining for senescence-associated-β-galactosidase (SA-β-gal) using the commercially available cellular senescence assay kit (Millipore Sigma, Billerica, MA) (14 (link), 15 (link)). Collagen deposition in liver sections (5 μm) was assessed by Sirius Red staining (10 different fields were analyzed from three different samples from three different animals) (14 (link), 15 (link)). Liver fibrosis was also measured by hydroxyproline levels in total liver samples using the hydroxyproline Assay Kit (14 (link)).

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Chen L., Zhou T., White T., O’Brien A., Chakraborty S., Liangpunsakul S., Yang Z., Kennedy L., Saxena R., Wu C., Meng F., Huang Q., Francis H., Alpini G, & Glaser S. (2021). The apelin-apelin receptor axis triggers cholangiocyte proliferation and liver fibrosis during mouse models of cholestasis. Hepatology (Baltimore, Md.), 73(6), 2411-2428.

Publication 2021

Dimension RxL Max Integrated Chemistry system cellular senescence assay kit

Alanine aminotransferase Alkaline phosphatase Animals Aspartate aminotransferase Assay Biliary Cellular senescence Collagen Dade Frozen sections Hydroxyproline Liver Liver fibrosis Paraffin Pathologist Serum Total bilirubin β galactosidase

Corresponding Organization : Texas A&M University

Other organizations : Indiana University – Purdue University Indianapolis, Richard L. Roudebush VA Medical Center, Southern Medical University

Top 5 similar protocols

Variable analysis

independent variables

Liver histology evaluation method (H&E staining)

dependent variables

Serum levels of alanine aminotransferase (ALT)
Serum levels of aspartate aminotransferase (AST)
Serum levels of alkaline phosphatase (ALK PHOS)
Serum levels of total bilirubin
Biliary senescence (SA-β-gal staining)
Collagen deposition (Sirius Red staining)
Liver fibrosis (hydroxyproline levels)

control variables

Paraffin-embedded liver sections (4–6 μm)
Frozen liver sections (10 μm)
Liver sections (5 μm)

positive controls

None specified

negative controls

None specified

Annotations

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