All animal procedures were performed in accordance with the Public Health Service Policy on Humane Care and Use of Laboratory Animals and were approved by the Northwestern University Institutional Animal Care and Use Committee. Eight to ten week old C57Bl/6 male and female mice (Jackson Laboratory) were used for antidepressant treatment experiments as well as DG virus injection experiments. For antidepressant and virus injection experiments, animals were allocated to experimental groups by randomly assigning each cage to an experimental treatment. BMPRII floxed mice (BMPRIIfx/fx)22 (link) were mated to mice containing tamoxifen-inducible Cre recombinase under the control of the Ascl1 promoter (Ascl1-CreER™)23 (link). To monitor Cre expression, these mice were crossed with Rosa-CAG-LSL-ZsGreen1 mice (RosaZG/ZG, Jackson Laboratory)24 (link). Tamoxifen-induced ablation of BMPRII was performed at 8–10 weeks of age in BMPRIIfx/fx; Ascl1-CreER™; RosaZG/ZG mice. BMPRII+/+; Ascl1-CreER™; RosaZG/ZG littermates were used as controls. All mice were housed 3–5 per cage under a 14:10 hour light:dark cycle with ad libitum access to food and water.