Plasma Biomarker Quantification Protocol

Tau and NfL concentrations were measured in plasma using the Simoa HD-1 Analyser and the “tau-2.0” and “NF-light” assay kits (Quanterix), according to manufacturer’s instructions and as described previously^{18 (link)}. Samples from each diagnostic group were assorted across different loading plates and assay runs. Samples were tested in duplicate, by taking two aliquots from the same plate well. Quality control parameters are detailed in the Supplementary Material. All “tau-2.0” testing kits were from a single batch. “NF-light” kits were taken from 2 separate batches due to a manufacturer issue: a full calibration sample set tested for each batch showed no significant variation between them.

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Thompson A.G., Anastasiadis P., Druyeh R., Whitworth I., Nayak A., Nihat A., Mok T.H., Rudge P., Wadsworth J.D., Rohrer J., Schott J.M., Heslegrave A., Zetterberg H., Collinge J., Jackson G.S, & Mead S. (2021). Evaluation of plasma tau and neurofilament light chain biomarkers in a 12-year clinical cohort of human prion diseases. Molecular Psychiatry, 26(10), 5955-5966.

Publication 2021

Simoa HD-1 Analyser NF-light” assay kits

Assay Diagnostic Light Plasma

Corresponding Organization :

Other organizations : MRC Prion Unit, University College London, UK Dementia Research Institute, University College London Hospitals NHS Foundation Trust, National Hospital for Neurology and Neurosurgery

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Variable analysis

independent variables

Tau concentration
NfL concentration

dependent variables

Tau concentration
NfL concentration

control variables

Measurement of tau and NfL concentrations using the Simoa HD-1 Analyser and the "tau-2.0" and "NF-light" assay kits (Quanterix), according to manufacturer's instructions and as described previously
Samples from each diagnostic group were assorted across different loading plates and assay runs
Samples were tested in duplicate, by taking two aliquots from the same plate well
All "tau-2.0" testing kits were from a single batch
"NF-light" kits were taken from 2 separate batches due to a manufacturer issue, with a full calibration sample set tested for each batch showing no significant variation between them

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