ChIP was performed as described previously [12 (link)]. Samples were immunoprecipitated with 2 μl anti-Red1 serum (Lot#16440, kind gift of N. Hollingsworth) or 2 μl anti-phospho-H2A-S129 antibody (Abcam #ab15083) per sample. Library preparation was performed as described [15 ]. Library quality was confirmed by Qubit HS assay and 2200 Tape Station. 100-bp single-end sequencing was performed on an Illumina NextSeq 500 instrument.
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