Detecting Carbonylated Proteins via OxyBlot

Carbonylated proteins were detected by the OxyBlot Kit (Millipore, S7150) as previously described,^{24 (link)} by using 15 μg of total proteins that were resolved in 12% SDS-polyacrylamide gels.

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Ciccarone F., Di Leo L., Lazzarino G., Maulucci G., Di Giacinto F., Tavazzi B, & Ciriolo M.R. (2019). Aconitase 2 inhibits the proliferation of MCF-7 cells promoting mitochondrial oxidative metabolism and ROS/FoxO1-mediated autophagic response. British Journal of Cancer, 122(2), 182-193.

Publication 2019

OxyBlot Kit

Polyacrylamide gels Proteins

Corresponding Organization : IRCCS Ospedale San Raffaele

Other organizations : University of Rome Tor Vergata, Saint Camillus International University of Health and Medical Sciences, Agostino Gemelli University Polyclinic

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Carbonylated proteins

control variables

Total proteins (15 μg)
12% SDS-polyacrylamide gels

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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