Single Cell RNA-Seq of Lung Cancer Cells

B[a]P and tricaprylin were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Chromium Single Cell 3′ v3 Reagent Kits (10x Genomics) and NextSeq 500/550 High Output sequencing reagent Kits v2 (150 cycles) (Illumina, San Diego, CA, USA) were used according to the manufacturer’s protocol [9 (link),10 (link),11 (link)]. LKR13 cells, a mouse lung adenocarcinoma line that expresses mutant KrasG12D on the SV129 background, were a generous gift from Dr. Jonathan M. Kurie (MD Anderson Cancer Center, Houston, TX, USA). LKR13-Luc cells were generated by transfecting LKR13 cells with a lentivirus expressing firefly luciferase (PLV-10064-200; Cellomics Technology, LLC, Halethrope, MD, USA). H2030-BrM3 cells were a generous gift from Dr. Joan Massage (Memorial Sloan Kettering Cancer Center, New York, NY, USA). All cell lines were grown in RPMI (Roswell Park Memorial Institute) 1640 medium (Cat# 11875; Thermo Fisher Scientific, Wiltham, MA, USA) supplemented with 10% fetal bovine serum at 37 °C in 5% carbon dioxide. A/J mice, FVB mice, and SV129 mice were purchased from Jackson Laboratory (Bar Harbor, ME, USA). Mito-HNK and Mito-LND were synthesized as previously described [4 (link),8 (link)] (Figure 1A).