Transmission Electron Microscopy Viral Imaging

The samples were prepared as described by Nikitin et al. [25 (link)] and examined either in a transmission electron microscope JEOL JEM-1400 TEM (JEOL, Japan) operating at 80 kV, or JEOL JEM-2100 (JEOL, Japan) operating at 200 kV. Images were recorded on an Olympus Quemesa digital camera under the control of iTEM software (Olympus Soft Imaging Solutions GmbH, Germany). The length and width of viral and virus-like particles were measured manually from digital prints using scientific image manipulation software ImageJ (National Institutes of Health, USA). To obtain the 3D reconstructions, the images were captured with an Ultrascan 1000XP 4x4 pixel CCD camera (Gatan) at x40,000 magnification with an underfocus of 1.5–2.8 um.

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Donchenko E.K., Pechnikova E.V., Mishyna M.Y., Manukhova T.I., Sokolova O.S., Nikitin N.A., Atabekov J.G, & Karpova O.V. (2017). Structure and properties of virions and virus-like particles derived from the coat protein of Alternanthera mosaic virus. PLoS ONE, 12(8), e0183824.

Publication 2017

JEM-1400 TEM Quemesa digital camera iTEM software

Prints Reconstructions Transmission electron microscope Virus particles

Corresponding Organization : Tel Hai Academic College

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Variable analysis

independent variables

Type of transmission electron microscope used (JEOL JEM-1400 TEM operating at 80 kV or JEOL JEM-2100 operating at 200 kV)

dependent variables

Length and width of viral and virus-like particles

control variables

Sample preparation method as described by Nikitin et al. [25]
Digital imaging using Olympus Quemesa camera and iTEM software
Image analysis using ImageJ software

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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