Expression of fixNOQP’-‘lacZ and fixK2′-‘lacZ fusions in B. diazoefficiens cells grown under microoxic conditions was analyzed by measuring β-Galactosidase activity. Cells cultivated for 48 h were first permeabilized and subsequently used for the assays, as previously described [55 ,57 (link)]. The absorbance at 420 nm of the enzymatic reactions and at 600 nm of the cultures was recorded in a plate reader (SUNRISE Absorbance Reader; TECAN, Männedorf, Switzerland) using the XFluor4 software (TECAN, Männedorf, Switzerland). These data were used to calculate the specific activity of β-Galactosidase in Miller units (MU).
Free full text: Click here
