HE staining was performed on 4-μm-thick sections according to the standard published protocols [23 (link)]. Sections were examined by experienced liver pathologists. The NAFLD activity score (NAS) was determined as described previously according to the following score [24 (link)]: steatosis (0 = < 5%; 1 = 5–33%; 2 = 33–66%; 3 = > 66%); intralobular inflammation (0 = no lesions; 1 = < 2 lesions/field of view; 2 = 2–4 lesions/field of view; 3 = > 4 lesions/field of view; and ballooning degeneration (0 = none; 1 = rare new balloon cells; 2 = common new balloon cells).
Frozen liver tissues were sectioned at 10-μm thickness on a cryostat (Leica CM1850, Germany), and fat cells were detected by an oil red O staining kit (Nanjing Jiancheng Bioengineering Institute, Jiangsu, China; batch number: 20180704) following the manufacturer’s instructions. Next, sections were analyzed under an inverted fluorescence microscope (Leica 37XB, Germany) and photographed.
Frozen liver tissues were sectioned at 10-μm thickness on a cryostat (Leica CM1850, Germany), and fat cells were detected by an oil red O staining kit (Nanjing Jiancheng Bioengineering Institute, Jiangsu, China; batch number: 20180704) following the manufacturer’s instructions. Next, sections were analyzed under an inverted fluorescence microscope (Leica 37XB, Germany) and photographed.
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