We used the continuous measurements of estimated glomerular filtration rate (eGFR) obtained in the baseline and wave 2, and the incident CKD (defined as GFR < 60 ml/min/1.73 m2 in wave 2, according with KDIGO 2012 (Clinical Practice Guideline for the Evaluation and Management of Chronic Kidney Disease) [9 ]. The GFR was estimated using the Chronic Kidney Disease Epidemiology Collaboration equation (CKD-EPI) [32 (link)] without correction for races, as detailed in Barreto et al. [15 (link)].
Creatinine was evaluated in serum samples by the kinetic method, according to Jaffé (Advia 1200; Siemens, Munich, Germany), by applying a conversion factor derived from the calibration sample for isotope dilution mass spectrometry, as recommended by the National Kidney Disease Education Program [33 (link)].
Creatinine was evaluated in serum samples by the kinetic method, according to Jaffé (Advia 1200; Siemens, Munich, Germany), by applying a conversion factor derived from the calibration sample for isotope dilution mass spectrometry, as recommended by the National Kidney Disease Education Program [33 (link)].
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