Western Blot Antibody Dilutions

Immunoblot analysis was performed as described in (9 (link)). Rabbit anti-GAPDH (Cell Signaling Technology: 2118L) was used at 1:2000. Anti-rabbit immunoglobulin G (IgG), horseradish peroxidase (HRP)–linked antibody (Cell Signaling Technology: 7074S) was used at 1:3000. Anti-mouse IgG, HRP-linked antibody (Cell Signaling Technology: 7076S) was used at 1:10,000. Rabbit anti-PARP (Cell Signaling Technology: 9452S) was used at 1:1500. Histone H3 antibody (Thermo Fisher Scientific; NB500-171) was used at 1:1000.

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Burke J.M., Gilchrist A.R., Sawyer S.L, & Parker R. (2021). RNase L limits host and viral protein synthesis via inhibition of mRNA export. Science Advances, 7(23), eabh2479.

Publication 2021

Rabbit anti-GAPDH Anti-rabbit immunoglobulin G (IgG), horseradish peroxidase (HRP)–linked antibody Rabbit anti-PARP Histone H3 antibody

Anti immunoglobulin Antibody Gapdh Histone h3 Horseradish peroxidase Immunoblot analysis Immunoglobulin g Mouse Rabbit

Corresponding Organization :

Other organizations : University of Colorado Boulder, Howard Hughes Medical Institute

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Variable analysis

independent variables

Rabbit anti-GAPDH (Cell Signaling Technology: 2118L)
Anti-rabbit immunoglobulin G (IgG), horseradish peroxidase (HRP)–linked antibody (Cell Signaling Technology: 7074S)
Anti-mouse IgG, HRP-linked antibody (Cell Signaling Technology: 7076S)
Rabbit anti-PARP (Cell Signaling Technology: 9452S)
Histone H3 antibody (Thermo Fisher Scientific; NB500-171)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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