Reprogramming Adult Cells to Induced Pluripotent Stem Cells

iPS-KYOU, has been obtained in the Shinya Yamanaka laboratory (Kyoto University, Japan) by retroviral reprogramming of adult female skin fibroblasts. The iPS-KYOU cell line was purchased from the ATCC cell bank (KYOU-DXR0109B, ACS-1023™, ATCC®, United States).
iPS-AFS17, human Induced Pluripotent Stem Cells, obtained by lentiviral reprogramming of amniotic fluid stem cells by Dashinimaev et al. (2017) (link).
iPS-DP human Induced Pluripotent Stem Cells, obtained by lentiviral reprogramming of dermal papilla cells (Muchkaeva et al., 2014 (link)).
iPS-DYP0730, human Induced Pluripotent Stem Cells, derived from dermal fibroblasts obtained from ATCC CCL-54 Detroit 532, a human Down syndrome cell line. The iPS-DYP0730 cell line was purchased from the ATCC cell bank (ACS-1003™, ATCC®, United States).
For all pluripotent stem cell passaging, we used ACCUTASE™ сell detachment solution (Stem Cell Technologies) and Rock-inhibitor Y-27632 (5 μM; Abcam), the plastic surface being pre-coated with Matrigel solution (1/40 in DMEM/F12) (Corning). The cells were cultured in mTeSR™1 medium (Stem Cell Technologies) at 37°C in a CO₂-incubator with 5% CO₂ and 100% humidity.

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Galiakberova A.A., Brovkina O.I., Kondratyev N.V., Artyuhov A.S., Momotyuk E.D., Kulmukhametova O.N., Lagunin A.A., Shilov B.V., Zadorozhny A.D., Zakharov I.S., Okorokova L.S., Golimbet V.E, & Dashinimaev E.B. (2023). Different iPSC-derived neural stem cells shows various spectrums of spontaneous differentiation during long term cultivation. Frontiers in Molecular Neuroscience, 16, 1037902.

Publication 2023

KYOU-DXR0109B ACS-1003 Rock-inhibitor Y-27632 Matrigel solution DMEM/F12 mTeSR™1 medium

Accutase Adult female Amniotic fluid Cell line Cells Cultured medium Down syndrome Fibroblasts Human Human induced pluripotent stem cells Humidity Ips cell Matrigel Papilla Pluripotent stem cell Retroviral Stem cell Y 27632

Corresponding Organization : Moscow Institute of Physics and Technology

Other organizations : Lomonosov Moscow State University, Federal Medical-Biological Agency, Mental Health Research Center of Russian Academy of Medical Sciences, Institute of Biomedical Chemistry, Bioinformatics Institute

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Variable analysis

independent variables

Reprogramming method (retroviral for iPS-KYOU, lentiviral for iPS-AFS17 and iPS-DP)
Cell types used for reprogramming (adult female skin fibroblasts for iPS-KYOU, amniotic fluid stem cells for iPS-AFS17, dermal papilla cells for iPS-DP, dermal fibroblasts from a Down syndrome cell line for iPS-DYP0730)

dependent variables

Generation of induced pluripotent stem cell (iPSC) lines (iPS-KYOU, iPS-AFS17, iPS-DP, iPS-DYP0730)

control variables

Cell culture conditions (ACCUTASE cell detachment solution, Rock-inhibitor Y-27632, Matrigel-coated plastic surface, mTeSR1 medium, 37°C, 5% CO2, 100% humidity)
Cell passaging protocol (ACCUTASE cell detachment solution, Rock-inhibitor Y-27632, Matrigel-coated plastic surface, mTeSR1 medium)

controls

Positive control: Not specified
Negative control: Not specified

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