Escherichia coli DH5α strain was purchased from TOYOBO, Japan. E. coli BL21 strain was used to express the GST fusion protein and was purchased from GE Healthcare, Japan [11 (link)].
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Protocol detail
Bacterial Strain Utilization for Protein Expression
E coli
Protein
Strain
Corresponding Organization : Kagawa Prefectural College of Health Sciences
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Variable analysis
independent variables
- Escherichia coli DH5α strain
- Escherichia coli BL21 strain
- Expression of GST fusion protein
- Not explicitly mentioned
- Positive control: Not specified
- Negative control: Not specified
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