BMP4-Induced Keratinocyte Response

For growth factor removal, mixed strain (129 × BL6) of wild-type newborn mouse skin was used as described to derive primary keratinocytes, which were expanded and used for experiments (between passages 4–6)47 (link). Cells were first grown to confluency. Then, cells were washed once with PBS and added serum-free media containing either recombinant BMP4 (R&D Systems) between 1 and 2 nM or its carrier (4 mM HCl in 0.1%BSA) only. Cells incubated for 6 and 12 h were harvested for RNA extractions.

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Lee J., Kang S., Lilja K.C., Colletier K.J., Scheitz C.J., Zhang Y.V, & Tumbar T. (2016). Signalling couples hair follicle stem cell quiescence with reduced histone H3 K4/K9/K27me3 for proper tissue homeostasis. Nature Communications, 7, 11278.

Publication 2016

recombinant BMP4

4 bsa Bmp4 Cells Growth factor Keratinocytes Mouse Newborn Serum free media Skin Strain

Corresponding Organization :

Other organizations : Cornell University

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Variable analysis

independent variables

Recombinant BMP4 (R&D Systems) between 1 and 2 nM
Carrier (4 mM HCl in 0.1% BSA) only

dependent variables

Gene expression (measured by RNA extraction)

control variables

Mixed strain (129 × BL6) of wild-type newborn mouse skin primary keratinocytes
Cells between passages 4-6
Cells grown to confluency
Cells incubated for 6 and 12 hours

controls

Positive control: Recombinant BMP4 (R&D Systems) between 1 and 2 nM
Negative control: Carrier (4 mM HCl in 0.1% BSA) only

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