Radiolabeled PET Tracers and Ligands Synthesis

2-[¹⁸F]fluoro-2-deoxy-D-glucose ([¹⁸F]FDG) was prepared in-house using a fully automated cassette-based synthesizer (FASTlab, GE Healthcare, Uppsala, Sweden) within the clinical routine production at the Vienna General Hospital, Austria. [¹¹C]SNAP-7941 was synthesized as reported elsewhere using an automated module (TRACERlab FC X Pro, GE Healthcare, Uppsala, Sweden) (25 (link)). All PET-tracers were physiologically formulated and quality-controlled prior to administration. The adrenergic receptor beta ligands carazolol, pindolol and (S)-propranolol hydrochloride were purchased from Sigma-Aldrich (St. Louis, USA). The ADRB3 agonist CL 316,243 was purchased from Tocris Bioscience (Bristol, UK). The unlabeled compounds FE@SNAP and SNAP-7941 were synthesized at the Department of Pharmaceutical Chemistry and at the Department of Organic Chemistry (University of Vienna, Austria). The radioligands 5,7-[³H](-)CGP-12177 and [¹²⁵I](-)Iodocyanopindolol were purchased from PerkinElmer, Inc. (Waltham, USA). All other reagents and cell culture supplies were purchased from standard commercial sources.