HeLa Cell Ultrastructure Imaged by SBF SEM

HeLa cells were were prepared, embedded in Durcupan and observed with SBF SEM following the method of the National Centre for Microscopy and Imaging Research (NCMIR) [80 ]. SBF SEM data were collected using a 3View2XP (Gatan, Pleasanton, CA, USA) attached to a Sigma VP SEM (Zeiss, Cambridge, UK). In total, five-hundred eighteen 518 images of 8192 × 8192 pixels were acquired. The voxel size was $10\times 10\times 50$ nm with intensity [0–255]. Figure 1a shows six EM images positioned within the 3D stack. Initially, the data were acquired at a higher bit-depth (32 bit or 16 bit), and after contrast/histogram adjustment, this was reduced to 8 bit [69 (link)]. Images are openly accessible via the EMPIAR [81 (link)] public image database (http://dx.doi.org/10.6019/EMPIAR-10094 Accessed on 28 May 2021).