Western Blot Analysis of Cell Signaling

Western blotting was performed using a SDS-PAGE Electrophoresis System according to the previous description [16 (link), 17 (link)] with antibodies specific for C-myc (Cell Signaling Technology, Beverly, MA, USA), CDK4 (Cell Signaling Technology), CDK6(Cell Signaling Technology), CyclinD1(Cell Signaling Technology), Rb (Cell Signaling Technology), p-Rb (Cell Signaling Technology), Caspase3 (Immunoway, USA), Cleaved Caspase3 (Cell Signaling Technology), Snail (Proteintech, USA), Slug (Proteintech), E-cadherin (Cell Signaling Technology), N-cadherin (Cell Signaling Technology), PI3K (Abclonal Technology), p-PI3K(Cell Signaling Technology), AKT(Cell Signaling Technology), p-AKT(Cell Signaling Technology), PTTG1(Cell Signaling Technology), β-Tublin (Cell Signaling Technology) and β-actin (Proteintech).Signals were detected using enhanced chemiluminescence reagents (Millipore, Schwalbach/Ts., Germany).