We followed a previously described method [39 (link)]. A total of 1 × 106 cells were incubated with FITC-conjugated CD44 and PE-conjugated CD24 (BD) and incubated at 4 °C for 20 min. The breast cancer cells were washed twice with 1XPBS and then analyzed by using a flow cytometer (Accuri C6, BD).
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