Mouse Hematopoietic Progenitor Phenotyping

For mouse phenotyping blood and femurs were collected from animals of different genotypes following standard procedure and processed as previously described16 (link). For flow analysis, samples were stained with Gr1-PE (Miltenyi Biotec, UK),CD11b (Mac1)-FITC (Becton Dickinson), CD71-PE (Becton Dickinson), Ter119-Fitc (Becton Dickinson). For analysis of progenitors population BM cells were lysed with Red Blood Cell buffer (NH4,Cl) and then enriched with the EasySep Hematopoietic Progenitor Cell Isolation Kit (StemCell technologies, UK) followed by staining with following antibodies Sca1-PB , c-KIT-Acy7, CD34-FITC, Flk2-PeCy5, Cd16/32-PE, CD150-PeCy7, CD48-APC.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Mupo A., Seiler M., Sathiaseelan V., Pance A., Yang Y., Agrawal A.A., Iorio F., Bautista R., Pacharne S., Tzelepis K., Manes N., Wright P., Papaemmanuil E., Kent D.G., Campbell P.C., Buonamici S., Bolli N, & Vassiliou G.S. (2016). Hemopoietic-specific Sf3b1-K700E knock-in mice display the splicing defect seen in human MDS but develop anemia without ring sideroblasts. Leukemia, 31(3), 720-727.

Publication 2016

Gr1-PE CD11b (Mac1)-FITC CD71-PE Ter119-Fitc EasySep Hematopoietic Progenitor Cell Isolation Kit CD34-FITC Cd16/32-PE

Animals Antibodies Blood Buffer C kit Cd11b Cd150 Cd71 Femurs Fitc Genotypes Hematopoietic progenitor cell Isolation Mouse Red blood cell Sca1 Stemcell

Corresponding Organization : University of Cambridge

Other organizations : Wellcome/MRC Cambridge Stem Cell Institute, European Bioinformatics Institute, Memorial Sloan Kettering Cancer Center

Top 5 similar protocols

Variable analysis

independent variables

Genotypes of the animals

dependent variables

Blood and femur samples collected from the animals
Cell populations analyzed by flow cytometry, including Gr1-positive, CD11b-positive, CD71-positive, Ter119-positive cells, as well as progenitor populations (Sca1-positive, c-KIT-positive, CD34-positive, Flk2-positive, CD16/32-positive, CD150-positive, CD48-positive cells)

control variables

Standard procedure for collecting blood and femur samples
Previously described processing methods for the samples (as referenced in the link provided)

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!