Evaluating s-cal14.2b Cytotoxicity in vitro

To ensure all in vitro studies were performed with non-cytotoxic treatment concentrations of s-cal14.2b, viability of NIT-1 and primary cells was measured in response to exogenous treatment with synthetic peptide [40 (link),41 (link)] s-cal14.2b. After three passages, 2 × 105 cells/well were grown in 96 well plates and treated with 0.1, 1, and 5 µg/mL s-cal14.2b for 24 h. Viability of NIT-1 and primary pancreatic and hepatic cells was then measured using the CellTiter 96^® Aqueous Cell Proliferation Assay (Promega, Madison, WI, USA). After incubating the cells with the assay reagent for 2 h at 37 °C, absorbance was measured at 490 nm using a Bio-Rad plate reader 680 Model (Bio-Rad, Hercules California, USA). The assays performed per triplicated in three independent assays.