Regulation of Cellular Signaling Pathways

All chemicals, including IS and ketoconazole, were from Sigma-Aldrich (St. Louis, MI, USA) unless otherwise stated. IS was used at concentrations of 2 μM and 4 μM, which correspond to the normal human serum concentration of IS [53 (link),95 (link),96 (link)]. The aryl hydrocarbon receptor (AHR) inhibitor, CH223191, was obtained from MedChemExpress (MCE, Monmouth Junction, NJ, USA) and was applied at a concentration of 10 μM. Pregnane X receptor (PXR) downstream signaling was inhibited using ketoconazole at a final concentration of 25 μM [75 (link),76 (link)].

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Sári Z., Mikó E., Kovács T., Boratkó A., Ujlaki G., Jankó L., Kiss B., Uray K, & Bai P. (2020). Indoxylsulfate, a Metabolite of the Microbiome, Has Cytostatic Effects in Breast Cancer via Activation of AHR and PXR Receptors and Induction of Oxidative Stress. Cancers, 12(10), 2915.

Publication 2020

CH223191 ketoconazole

Aryl hydrocarbon receptor Ch223191 Human Ketoconazole Pregnane x receptor Serum

Corresponding Organization : University of Debrecen

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Variable analysis

independent variables

IS concentration at 2 μM and 4 μM
AHR inhibitor CH223191 at 10 μM
PXR inhibitor ketoconazole at 25 μM

dependent variables

Outcomes not explicitly mentioned

control variables

All chemicals, including IS and ketoconazole, were from Sigma-Aldrich (St. Louis, MI, USA) unless otherwise stated

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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