CD19+ B cell counts were determined on whole blood after red blood cell lysis using mAbs specific for CD19 (BD Bioscience, San Jose, CA). Absolute numbers of B cells were calculated based on patient white blood count and the immunophenotypic data. Phenotyping analysis of B cells was performed by labeling blood lymphocytes with titrated volumes of anti-CD24 FITC (BD biosciences), anti-CD38 PE (Beckman Coulter, Fullerton, CA), anti-CD27APC/Cy7 (Biolegend, San Diego, CA). Cells were analyzed using a FACSVerse flow cytometer (BD Biosciences). The flow cytometer calibrated by methods described previously12 (link).
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Quantification of CD19+ B Cells
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Corresponding Organization : Columbia University Irving Medical Center
Other organizations : First Hospital of Jilin University, Jilin University, The Affiliated Hospital to Changchun University of Chinese Medicine
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Variable analysis
independent variables
- CD19+ B cell counts
- Absolute numbers of B cells
- Patient white blood count
- Immunophenotypic data
- Calibration of the FACSVerse flow cytometer by methods described previously
- The flow cytometer was calibrated by methods described previously
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