Macrophage Polarization and p38 MAPK Inhibition

BMDMs were isolated from 7- to 8-week-old mice and cultured as described previously to obtain naïve M0 macrophages (Dai et al., 2017 (link)). For M1 polarization, BMDMs were stimulated with 10 ng/ml LPS (Sigma Aldrich) for 24 h. For M2 polarization, BMDMs were stimulated with 10 ng/ml IL-4 (BioLegend) for 24 h. Pharmacologic inhibition of p38 MAPK (SB203580; Sigma Aldrich) was achieved by pretreating BMDMs with 10 µM SB203580 for 1 h before addition of LPS or IL-4.

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Wang B., Lim C.B., Yan J., Li L., Wang J., Little J.B, & Yuan Z.M. (2020). MDMX phosphorylation-dependent p53 downregulation contributes to an immunosuppressive tumor microenvironment. Journal of Molecular Cell Biology, 12(9), 713-722.

Publication 2020

LPS IL-4 SB203580

Il 10 Inhibition Macrophages Mice P38 mapk Sb203580

Corresponding Organization :

Other organizations : Institute of Modern Physics, Chinese Academy of Sciences, University of Chinese Academy of Sciences

Top 5 similar protocols

Variable analysis

independent variables

Macrophage polarization treatment
Pharmacologic inhibition of p38 MAPK

dependent variables

Phenotypic characteristics of macrophages

control variables

Macrophage isolation from 7- to 8-week-old mice
Culture conditions for obtaining naïve M0 macrophages

controls

Naïve M0 macrophages (unstimulated control)
Pharmacologic inhibition of p38 MAPK (untreated control)

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