Cells were seeded at 1.0×105 cells/well in a 24-well culture plate and incubated for 48 h. Cells were treated with Hoechst 33342 cell-permeable nuclear counterstain dye for 10 min, and images were acquired using a fluorescence microscope equipped with a CoolSnap-Pro color digital camera (Media Cybernetics, Rockville, MD, USA). The apoptotic index was calculated using a formula as described previously (Piao et al., 2019 (link)).
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Apoptotic Index Determination by Fluorescence Microscopy
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Apoptotic
Camera
Cell
Cells culture
Fluorescence microscope
Hoechst 33342
Permeable
Corresponding Organization :
Other organizations : Jeju National University Hospital, Jeju National University
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Variable analysis
independent variables
- Hoechst 33342 cell-permeable nuclear counterstain dye
- Apoptotic index
- Cells seeded at 1.0×10^5 cells/well in a 24-well culture plate
- Cells incubated for 48 h
- No positive or negative controls were explicitly mentioned.
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