Intracellular GSH changes were measured using Ellman’s reagent (DTNB; Sigma-Aldrich, St. Louis, MO, USA) employing the protocol proposed by Tan et al. [81 (link)]. The absorbance was measured at 405 nm using a microplate reader (BioSan, Riga, Latvia). The concentration of free thiols in the samples was calculated using a GSH (Sigma-Aldrich, St. Louis, MO, USA) standard curve.
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