Histopathological Examination of Rat Organ Tissues

Biopsy (portions) of the liver and kidney tissues from each rat was taken cautiously. After adding 10% formalin, the tissues in paraffin are fixed and stained with hematoxylin and eosin (H&E) dye in the EPHI pathology laboratory. The control and treatment animal organ slides were subjected for examination through the binocular light microscope (Olympus CX41, Japan) at a magnification of 20x and 40x. The micrographs of organ histopathology were analyzed and interpreted by a pathologist according to similar method used in [30 (link)].

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Habte K., Azene M., Chanyalew Y., Girma S., Bashea C., Yehualshet A., Behailu G., Abebe A, & Tessema M. (2023). Nutrient Density and Microbial Safety of Open-Air-Dried Beef Meat and Its Biochemical and Organ Histopathology Effects in Albino Rats: A Promising Ingredient for Complementary Food Formulation. International Journal of Food Science, 2023, 2202312.

Publication 2023

Animal organ Biopsy Eosin Formalin Kidney Light microscope Liver Paraffin Pathologist Tissues

Corresponding Organization : Ethiopian Public Health Institute

Other organizations : Addis Ababa University, International Trachoma Initiative

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Variable analysis

independent variables

Animal treatment

dependent variables

Histopathological changes in liver and kidney tissues

control variables

Species of animals used (rats)
Tissue processing (fixation in 10% formalin, paraffin embedding, H&E staining)
Microscope used (Olympus CX41, Japan) and magnification (20x and 40x)
Method of histopathological examination and interpretation by a pathologist

controls

Control animals (no treatment)

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