Serological Detection of Zika Virus

Serology for ZIKV was performed with the “Anti-Zika Virus ELISA (IgG)” kit (EI 2668–9601 G, EUROIMMUN Schweiz AG, Luzern, Switzerland), according to the manufacturer’s specifications. Confirmation of positive samples was performed using a custom designed flavivirus mosaic Indirect Immunofluorescence Test (IIFT) (EUROIMMUN Schweiz AG, Luzern, Switzerland), in which cells infected with Zika, Dengue (I-IV), West Nile, Yellow fever and Japanese encephalitis viruses were used to detect their antigens.

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Chui Y.C., Baud D., Fahmi A., Zumkehr B., Vouga M., Pomar L., Musso D., Thuong B.C., Alves M.P, & Stojanovic M. (2023). Absence of Zika virus among pregnant women in Vietnam in 2008. Tropical Diseases, Travel Medicine and Vaccines, 9, 4.

Publication 2023

Antigens Cells Dengue Elisa Flavivirus Indirect immunofluorescence Japanese encephalitis viruses Yellow fever Zika

Corresponding Organization :

Other organizations : University of Lausanne, Aix-Marseille Université, Assistance Publique Hôpitaux de Marseille, Institut de Recherche pour le Développement, Viet Duc Hospital

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Detection of anti-Zika virus IgG antibodies

control variables

None explicitly mentioned

controls

Positive control: Cells infected with Zika, Dengue (I-IV), West Nile, Yellow fever and Japanese encephalitis viruses used to detect their antigens
Negative control: Not mentioned

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