Cell Growth Assay for LCL and JRS Cells

For cell growth analysis, a 2 ml aliquot of LCL wash plated at 5 × 10⁴ cells/ml per well in 12‐well plates and JRS cells were plated at 5 × 10⁴ cells per well in 6‐well plates. Following 0–96 h incubations in medium with or without Asn (RPMI for LCL, DMEM for JRS cells), cells were collected to determine viable cell numbers using the Applied Biosystems Countess II FL automated cell counter per the manufacturer's trypan blue staining protocol. All reported values are trypan‐excluding, viable cells only.

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Staklinski S.J., Chang M.C., Ahrens‐Nicklas R.C., Kaur S., Stefanatos A.K., Dudenhausen E.E., Merritt M.E, & Kilberg M.S. (2023). Characterizing asparagine synthetase deficiency variants in lymphoblastoid cell lines. JIMD Reports, 64(2), 167-179.

Publication 2023

Countess II FL

Cells Trypan Trypan blue

Corresponding Organization : Children's Hospital of Philadelphia

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Variable analysis

independent variables

Presence or absence of Asn (RPMI for LCL, DMEM for JRS cells)

dependent variables

Viable cell numbers

control variables

Cell line (LCL and JRS cells)
Plating density (5 × 10^4 cells/ml per well)
Incubation duration (0-96 h)
Cell counting method (Applied Biosystems Countess II FL automated cell counter with trypan blue staining)

controls

Positive control: Cells incubated with Asn
Negative control: Cells incubated without Asn

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