Measuring Soluble Biomarkers in CSF and Plasma

Levels of soluble biomarkers were measured in CSF and plasma via a combination of Luminex-based custom assays and ELISAs, as per manufacturers’ protocol and as described previously [31 (link)]. IP-10/CXCL10 and IL-10 were measured using the Luminex based Milliplex Map Human Cytokine / Chemokine Panel (Millipore Sigma, St. Louis MO). Soluble CD163 was measured via the Bio-Plex Pro Human Inflammation Panel 1 (Bio-Rad Laboratories, Hercules CA). Neopterin was measured by chemiluminescent detection ELISA (Genway Biotech, San Diego CA). Data was collected on a FlexMap 3D reader (Bio-Rad Laboratories, Hercules CA) or VersaMax plate reader (Molecular Devices, Sunnyvale CA) and analyzed in Prism v.9 for Mac OS X (GraphPad, La Jolla CA) using 4-parameter fit standard curves.

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Sharma V., Creegan M., Tokarev A., Hsu D., Slike B.M., Sacdalan C., Chan P., Spudich S., Ananworanich J., Eller M.A., Krebs S.J., Vasan S, & Bolton D.L. (2021). Cerebrospinal fluid CD4+ T cell infection in humans and macaques during acute HIV-1 and SHIV infection. PLoS Pathogens, 17(12), e1010105.

Publication 2021

Bio-Plex Pro Human Inflammation Panel 1 FlexMap 3D reader VersaMax

Assays Biomarkers Cd163 Chemokine Cytokine Devices Elisa Human Il 10 Inflammation Neopterin Plasma Prism

Corresponding Organization : Henry M. Jackson Foundation

Other organizations : Armed Forces Research Institute of Medical Science, HIV Netherlands Australia Thailand Research Collaboration, Yale University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Levels of soluble biomarkers IP-10/CXCL10, IL-10, and soluble CD163 in CSF and plasma
Neopterin levels in CSF and plasma

control variables

None explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

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