The presence of human stromal cells in the kidney, lung, liver, and heart of RMR rats was determined as previously described17 (link)
. Tissue sections were incubated for 1 hour with a 1% bovine serum albumin blocking solution, followed by incubation with Alexa Fluor 488 conjugated anti-human nuclear antigen (HNA) antibody (1:50; clone 235 -1, Merck Millipore) overnight at 4 °C. Cell nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI; Merck Millipore), and the tissue structures were marked using Rhodamine-labeled wheat germ agglutinin (WGA, 1:400; Vector Laboratories, Burlingame, CA, USA).
. Tissue sections were incubated for 1 hour with a 1% bovine serum albumin blocking solution, followed by incubation with Alexa Fluor 488 conjugated anti-human nuclear antigen (HNA) antibody (1:50; clone 235 -1, Merck Millipore) overnight at 4 °C. Cell nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI; Merck Millipore), and the tissue structures were marked using Rhodamine-labeled wheat germ agglutinin (WGA, 1:400; Vector Laboratories, Burlingame, CA, USA).
Free full text: Click here
