Retinal Antibody and Isolectin Labeling

Antibody and isolectin labeling of retinas was performed as previously described (Stefater et al., 2011 (link)) with the following: Alexa 488-Isolectin IB4 (Life Technologies, I21411; 1:1000), Crim1 (homemade rabbit antiserum), collagen IV (rabbit; Abcam, ab19808; 1:500), BrdU (mouse; Dako, M0744; 1:100), VE-cadherin (rat; BD Biosciences, 555289; 1:100), VE-cadherin (goat; Santa Cruz, sc-6458; 1:200), CD31 (rat; BD Biosciences, 550274; 1:100), active caspase 3 (rabbit; R&D Systems, AF835; 1:100), podocalyxin (goat; R&D Systems, AF1556; 1:200), PDGFRα (goat; R&D Systems, AF1062; 1:100), desmin (rabbit; Abcam, ab15200; 1:500) and smooth muscle actin (mouse; Sigma, C6198; 1:200).

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Fan J., Ponferrada V.G., Sato T., Vemaraju S., Fruttiger M., Gerhardt H., Ferrara N, & Lang R.A. (2014). Crim1 maintains retinal vascular stability during development by regulating endothelial cell Vegfa autocrine signaling. Development (Cambridge, England), 141(2), 448-459.

Publication 2014

VE-cadherin AF835 AF1556 AF1062 ab15200

Actin Antibody Antiserum Brdu Caspase 3 Collagen iv Desmin Goat Isolectin Mouse Pdgfrα Podocalyxin Rabbit Retinas Smooth muscle Ve cadherin

Corresponding Organization :

Other organizations : Cincinnati Children's Hospital Medical Center, University of Cincinnati, National Defense Medical College, Saitama Prefecture, University College London, Cancer Research UK, University of California, San Diego, University of Cincinnati Medical Center

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Protocol cited in 1 other protocol

Variable analysis

independent variables

Antibody and isolectin labeling

dependent variables

Retinal labeling

control variables

Alexa 488-Isolectin IB4 (Life Technologies, I21411; 1:1000)
Crim1 (homemade rabbit antiserum)
Collagen IV (rabbit; Abcam, ab19808; 1:500)
BrdU (mouse; Dako, M0744; 1:100)
VE-cadherin (rat; BD Biosciences, 555289; 1:100)
VE-cadherin (goat; Santa Cruz, sc-6458; 1:200)
CD31 (rat; BD Biosciences, 550274; 1:100)
Active caspase 3 (rabbit; R&D Systems, AF835; 1:100)
Podocalyxin (goat; R&D Systems, AF1556; 1:200)
PDGFRα (goat; R&D Systems, AF1062; 1:100)
Desmin (rabbit; Abcam, ab15200; 1:500)
Smooth muscle actin (mouse; Sigma, C6198; 1:200)

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