Immunohistochemical Analysis of SIRT1, TFAM, COXIV, and TOMM20

Immunohistochemical staining was performed on 5-μm sections of paraformaldehyde- fixed, paraffin-embedded tumor samples, adjacent nontumoral liver tissue, normal liver tissue and mouse lung tissue. The details of the immunohistochemistry were described previously [28 (link)]. The slides were incubated with mouse anti-SIRT1 (Abcam), rabbit anti-TFAM (Abcam), rabbit anti-COXIV (Cell Signaling Technology, Danvers, MA, USA) and rabbit anti-TOMM20 (Abcam) primary antibodies. The mouse lung tissue sections (5-μm thick) were stained with hematoxylin and eosin (H&E) so that tumor nodules could be observed. The slides were evaluated under a light microscope (Leica, DM6000M).

Free full text: Click here

Li Y., Xu S., Li J., Zheng L., Feng M., Wang X., Han K., Pi H., Li M., Huang X., You N., Tian Y., Zuo G., Li H., Zhao H., Deng P., Yu Z., Zhou Z, & Liang P. (2016). SIRT1 facilitates hepatocellular carcinoma metastasis by promoting PGC-1α-mediated mitochondrial biogenesis. Oncotarget, 7(20), 29255-29274.

Publication 2016

mouse anti-SIRT1 rabbit anti-TFAM rabbit anti-COXIV rabbit anti-TOMM20 DM6000M

Antibodies Embedded paraffin Eosin Immunohistochemistry Liver Lung Microscope light Mouse Paraformaldehyde Rabbit Sirt1 Tfam Tissue Tomm20 Tumor

Corresponding Organization :

Other organizations : Army Medical University

Top 5 similar protocols

Protocol cited in 1 other protocol

Variable analysis

independent variables

Immunohistochemical staining on 5-μm sections of paraformaldehyde-fixed, paraffin-embedded tumor samples, adjacent nontumoral liver tissue, normal liver tissue and mouse lung tissue

dependent variables

Immunohistochemical staining of SIRT1, TFAM, COXIV and TOMM20 proteins

control variables

Mouse lung tissue sections (5-μm thick) stained with hematoxylin and eosin (H&E) to observe tumor nodules

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!