Establishment and Characterization of Lapatinib-Resistant Breast Cancer Cell Line

The human breast cancer cell line UACC812, originally purchased from the American Type Culture Collection (Manassas, VA, USA), was cultured as previously reported (6 (link)). UACC812 cells resistant to lapatinib (LR) were derived from parental cells in vitro and maintained as previously described (6 (link)). Both parental and LR cell lines were authenticated by DNA sequencing and immediately frozen in our laboratory. Upon thawing, cell morphology, signaling, and sensitivity/resistance to L were maintained. L LC Laboratories, (Woburn, MA, USA) and ABT-737 (Selleckchem, Houston, Tx, USA) were dissolved in dimethyl sulfoxide (DMSO). Fulvestrant (Ful), AstraZeneca (London, UK), was prepared with ethanol.

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Giuliano M., Hu H., Wang Y.C., Fu X., Nardone A., Herrera S., Mao S., Contreras A., Gutierrez C., Wang T., Hilsenbeck S.G., De Angelis C., Wang N.J., Heiser L.M., Gray J.W., Lopez-Tarruella S., Pavlick A.C., Trivedi M.V., Chamness G.C., Chang J.C., Osborne C.K., Rimawi M.F, & Schiff R. (2015). Upregulation of ER signaling as an adaptive mechanism of cell survival in HER2-positive breast tumors treated with anti-HER2 therapy. Clinical cancer research : an official journal of the American Association for Cancer Research, 21(17), 3995-4003.

Publication 2015

UACC812 ABT-737

Abt 737 Breast cancer cell line Cell lines Cells Dimethyl sulfoxide Ethanol Frozen Fulvestrant Human Lapatinib Parental Sensitivity

Corresponding Organization : Baylor College of Medicine

Other organizations : Federico II University Hospital, Oregon Health & Science University, Hospital General Universitario Gregorio Marañón, University of Houston, Houston Methodist, Methodist Hospital

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Variable analysis

independent variables

Lapatinib (L)

dependent variables

Cell morphology
Cell signaling
Sensitivity/resistance to L

control variables

Cell line (UACC812 parental and UACC812 lapatinib-resistant (LR) cell lines)

controls

Positive control: UACC812 parental cell line
Negative control: Not explicitly mentioned

Annotations

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