Lycium barbarum Polysaccharide Immunomodulation

LBP was prepared from Lycium barbarum by our laboratory as described previously [23 (link)]. The total sugar and protein content was 70.13% and 19.30%, respectively. The fractions with molecular weight range from 40 kDa to 350 kDa were prepared and used in this study. Mouse 1x lymphocyte separation medium was purchased from Dakewe Biotech Co. Ltd. (Shenzhen, China). PE/CY7-anti-mouse CD3, FITC-anti-mouse CD4, PE-anti-mouse PD-1, PE/CY5-anti-mouse CD25, and purified CD8 antibody were purchased from BioLegend. Purified CD3 antibody was purchased from Affinity Bioscience. Propidium iodide (PI), collagenase type IV, and DNase I were purchased from Sigma. Mouse IL-10 and mouse TGF-β1 ELISA kit were purchased from MULTI SCIENCE (Hangzhou, China).