Immunohistochemical Analysis of Hepatic IL-1β

Liver tissues were fixed in 4% (v/v) paraformaldehyde (PFA) in PBS and embedded with paraffin, which were then sliced into tissue sections (4 μM) and mounted on glass slides. These tissue slides were stained with goat anti-IL-1β antibody (1:100, R&D Systems) overnight at 4°C after a 20 min wash with 3% H₂O₂ and 30 min blocking with 10% serum and then probed with anti-goat Ig-G second antibody labeled with HRP according to the protocols described previously [32 (link), 40 (link)]. Negative controls were prepared without the primary antibodies. The area percentage of the positive staining were calculated in Image Pro Plus 6.0 software.

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Meng N., Xia M., Lu Y.Q., Wang M., Boini K.M., Li P.L, & Tang W.X. (2016). Activation of NLRP3 inflammasomes in mouse hepatic stellate cells during Schistosoma J. infection. Oncotarget, 7(26), 39316-39331.

Publication 2016

goat anti-IL-1β antibody

Anti antibody Anti ig g Antibodies Antibody Embedded paraffin Goat H2o2 Il 1β Liver Paraformaldehyde Serum Tissue

Corresponding Organization : Tongji Hospital

Other organizations : Virginia Commonwealth University

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Variable analysis

independent variables

Staining with goat anti-IL-1β antibody (1:100, R&D Systems) overnight at 4°C

dependent variables

Area percentage of the positive staining

control variables

Liver tissues were fixed in 4% (v/v) paraformaldehyde (PFA) in PBS and embedded with paraffin
Tissue sections (4 μM) were mounted on glass slides
Tissue slides were treated with 3% H2O2 for 20 min and blocked with 10% serum for 30 min
Tissue slides were probed with anti-goat Ig-G second antibody labeled with HRP

positive controls

Not explicitly mentioned

negative controls

Tissue slides prepared without the primary antibodies

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