Bones were scanned using high-resolution μCT (Xradia, MicroXCT-200, Carl Zeiss X-ray Microscopy, Inc., Pleasanton, CA) with 1.1035 μm nominal voxel size (x-ray tube potential = 80 kVp, current = 100 μA), filter for 20x of 0.14–0.08 transmission (LE #6). The scan region of interest was a 1 mm3 cubic volume that contained both cortical and trabecular regions on the anterior side of the proximal tibia. All analyses were performed using Amira analysis software (Thermo Fisher Scientific, Berlin, Germany). Lacunae in trabecular bone and cortical bone were analyzed separately. Lacunae were identified as porosities with a volume size of 100–800 μm3 [17 (link), 18 (link)]. Total lacunar volume per bone volume and average lacunar volume were determined for each of the trabecular and cortical regions.
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High-Resolution μCT Analysis of Bone Lacunae
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Corresponding Organization : University of California, Davis
Other organizations : Lawrence Livermore National Laboratory, University of California, San Francisco
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Variable analysis
independent variables
- Bone scanning using high-resolution μCT
- Total lacunar volume per bone volume
- Average lacunar volume
- Scan region of interest (1 mm^3 cubic volume containing both cortical and trabecular regions on the anterior side of the proximal tibia)
- Voxel size (1.1035 μm nominal)
- X-ray tube potential (80 kVp)
- X-ray tube current (100 μA)
- Filter for 20x of 0.14–0.08 transmission (LE #6)
- No positive or negative controls were explicitly mentioned in the provided information.
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