Protein stability was assessed by differential scanning fluorimetry as previously reported.39 (link) Briefly, bovine rhodopsin (Uniprot entry P02699) and the green pigment were diluted in 20 mM HEPES (pH 7.0), 50 mM NaCl, and 0.02% DDM to a concentration of 6 μM and assayed in the presence of 2 μM reporter dye, BODIPY FL l -cystine (ThermoFisher, Waltham, MA). Melting temperatures were determined in triplicate using a Boltzmann fit performed with SigmaPlot version 11.0.
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Differential Scanning Fluorimetry for Protein Stability
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Variable analysis
independent variables
- Bovine rhodopsin (Uniprot entry P02699)
- The green pigment
- Protein stability
- Melting temperatures
- 20 mM HEPES (pH 7.0)
- 50 mM NaCl
- 0.02% DDM
- 2 μM reporter dye, BODIPY FL l-cystine
- Melting temperatures were determined in triplicate
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