Quantifying Clostridium difficile Toxins

Secreted CDTa/b was assessed by Western blot analysis of 48-hour culture-free supernatants exactly as described previously [17 (link)], using an HRP-Chicken anti-Clostridium difficile Binary Toxin Subunit A or B antibody (Gallus-Immunotech, Shirley, MA).

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Simpson M., Bilverstone T., Leslie J., Donlan A., Uddin M.J., Petri W.A., Marin N., Kuehne S., Minton N.P, & Petri WA J.r. (2023). Clostridioides difficile Binary Toxin Binding Component Increases Virulence in a Hamster Model. Open Forum Infectious Diseases, 10(3), ofad040.

Publication 2023

Antibody Cdta Chicken Clostridium difficile toxin a Subunit Western blot analysis

Corresponding Organization : University of Virginia

Other organizations : Engineering and Physical Sciences Research Council, University of Nottingham, Biotechnology and Biological Sciences Research Council, Nottingham University Hospitals NHS Trust, Nottingham Biomedical Research Centre, University of Birmingham

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Variable analysis

independent variables

None explicitly mentioned.

dependent variables

Secreted CDTa/b (Clostridium difficile Binary Toxin Subunit A or B) assessed by Western blot analysis

control variables

Not explicitly mentioned.

controls

None explicitly mentioned.
None explicitly mentioned.

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