Cells were grown to confluence in a 12 well-plate at 24 h after seeding (5 x 105 cells/well), were treated with PHMG at the concentration of 1 μg/mL, 5 μg/mL, 10 μg/mL, and 25 μg/mL, and further incubated with 40 μM of H2DCFDA (Invitrogen, CA, USA) for 1 h. At the end of H2DCFDA incubation, cells were washed with phosphate-buffered saline (PBS), and were visualized with a fluorescent microscope (Nikon, Tokyo, Japan) [14 (link)].
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PHMG-induced Oxidative Stress Measurement
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Other organizations : Dongduk Women's University
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Variable analysis
independent variables
- PHMG concentration: 1 μg/mL, 5 μg/mL, 10 μg/mL, and 25 μg/mL
- Reactive oxygen species (ROS) levels measured using H2DCFDA fluorescence
- Cell density: 5 x 10^5 cells/well
- Incubation time with H2DCFDA: 1 h
- No positive or negative controls were explicitly mentioned in the provided information.
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