Reagents for Lubrication Testing
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Corresponding Organization :
Other organizations : University of California, San Diego, Colorado State University, Stanford University
Variable analysis
- Hyaluronan (HA) in a 4,000 kDa form (Healon®, Advanced Medical Optics, Santa Ana, CA)
- Antibody to PRG4 (anti-Lubricin from AbCam, Cambridge, MA)
- Nonspecific rabbit IgG (from Pierce, Rockford, IL)
- Mouse anti-rabbit IgG secondary antibody (from Jackson ImmunoResearch, West Grove, PA)
- Streptomyces hyaluronidase (from Seikagaku, Tokyo, Japan)
- SeaKem® gold agarose (from Lonza, Rockland, ME)
- 50X TAE (2M Tris, 0.5M EDTA) electrophoresis buffer (from Life Technologies, Carlsbad, CA)
- Hybond™-P polyvinylidene difluoride (PVDF) membrane for Western blotting (from GE Healthcare, Piscataway, NJ)
- Stains-All (from Sigma-Aldrich, St. Louis, MO)
- Lubrication testing of materials
- Reagents for lubrication testing materials were as described previously in the cited references
- None specified
- Nonspecific rabbit IgG
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