Reagents for Lubrication Testing

Reagents for lubrication testing materials were as described previously,^{2 (link),6 (link),33 (link)} including hyaluronan (HA) in a 4,000 kDa form (Healon®, Advanced Medical Optics, Santa Ana, CA). The antibody to PRG4 was anti-Lubricin from AbCam (Cambridge, MA);^{2 (link)} nonspecific rabbit IgG was from Pierce (Rockford, IL); mouse anti-rabbit IgG secondary antibody was from Jackson ImmunoResearch (West Grove, PA). Streptomyces hyaluronidase was from Seikagaku (Tokyo, Japan). SeaKem® gold agarose was from Lonza (Rockland, ME); 50X TAE (2M Tris, 0.5M EDTA) electrophoresis buffer was from Life Technologies (Carlsbad, CA); Hybond™-P polyvinylidene difluoride (PVDF) membrane for Western blotting was from GE Healthcare (Piscataway, NJ). Stains-All was from Sigma-Aldrich (St. Louis, MO).

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Grissom M.J., Temple-Wong M.M., Adams M.S., Tom M., Schumacher B.L., McIlwraith C.W., Goodrich L.R., Chu C.R, & Sah R.L. (2014). Synovial Fluid Lubricant Properties Are Transiently Deficient After Arthroscopic Articular Cartilage Defect Repair With Platelet-Enriched Fibrin Alone and With Mesenchymal Stem Cells. Orthopaedic Journal of Sports Medicine, 2(7), 2325967114542580.

Publication 2014

anti-Lubricin mouse anti-rabbit IgG secondary antibody Streptomyces hyaluronidase SeaKem® gold agarose Stains-All

Agarose Anti igg Antibody Buffer Edta Electrophoresis Gold Healon Hyaluronan Hyaluronidase Lubrication Lubricin Membrane Mouse Optics Polyvinylidene difluoride Rabbit Stains all Streptomyces Tris

Corresponding Organization :

Other organizations : University of California, San Diego, Colorado State University, Stanford University

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Variable analysis

independent variables

Hyaluronan (HA) in a 4,000 kDa form (Healon®, Advanced Medical Optics, Santa Ana, CA)
Antibody to PRG4 (anti-Lubricin from AbCam, Cambridge, MA)
Nonspecific rabbit IgG (from Pierce, Rockford, IL)
Mouse anti-rabbit IgG secondary antibody (from Jackson ImmunoResearch, West Grove, PA)
Streptomyces hyaluronidase (from Seikagaku, Tokyo, Japan)
SeaKem® gold agarose (from Lonza, Rockland, ME)
50X TAE (2M Tris, 0.5M EDTA) electrophoresis buffer (from Life Technologies, Carlsbad, CA)
Hybond™-P polyvinylidene difluoride (PVDF) membrane for Western blotting (from GE Healthcare, Piscataway, NJ)
Stains-All (from Sigma-Aldrich, St. Louis, MO)

dependent variables

Lubrication testing of materials

control variables

Reagents for lubrication testing materials were as described previously in the cited references

positive controls

None specified

negative controls

Nonspecific rabbit IgG

Annotations

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