Modified mRNA Production for HIV-1 Vaccine
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Corresponding Organization : University of Pennsylvania
Other organizations : National Institute of Allergy and Infectious Diseases, National Institutes of Health, Acuitas Therapeutics (Canada)
Variable analysis
- Transcription using T7 RNA polymerase on linearized plasmids encoding codon-optimized CH848 10.17DT gp160s, CH848 10.17DT SOSIP trimers, or CH848 10.17DT trimer-ferritin NPs
- Use of one-methylpseudouridine (m1Ψ)-5'-triphosphate instead of UTP to produce nucleoside-modified mRNAs
- Capping method (ScriptCap m7G capping system and ScriptCap 2′-O-methyl-transferase kit for CH848 10.17DT SOSIPv4.1 trimer and CH848 10.17DT SOSIPv5.2.8 trimer mRNAs, and CleanCap trinucleotide cap1 analog for all other in vitro transcribed mRNAs)
- Not explicitly mentioned
- Purification of all mRNAs by cellulose purification
- Storage of all mRNAs frozen at -20 °C
- Not mentioned
- Not mentioned
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