Bacterial 16S rDNA Amplification and Identification

The LAB were grown overnight and the genomic DNA was extracted from 1.5 ml cultures using a bacterial genome extraction kit (Tiangen, China) following the manufacturer's protocols. The identification of isolates was analyzed based on the 16S rDNA gene amplification with primer pair: 16S rDNA-F (5′-AGA GTT TGA TCC ATG GCT CAG-3′)/16S rDNA-R (5′-AAG GAG GTG ATC CAG CC-3′). Then PCR was performed using a previously described method (24 (link)). The sequences for the amplified 16S rDNA were searched using Blast in the NCBI databases to compare with the registered sequences.

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Li M., Wang Y., Cui H., Li Y., Sun Y, & Qiu H.J. (2020). Characterization of Lactic Acid Bacteria Isolated From the Gastrointestinal Tract of a Wild Boar as Potential Probiotics. Frontiers in Veterinary Science, 7, 49.

Publication 2020

bacterial genome extraction kit

Bacterial genome Gene amplification Genomic Primer Rdna

Corresponding Organization : Chinese Academy of Agricultural Sciences

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Variable analysis

independent variables

Primer pair: 16S rDNA-F (5′-AGA GTT TGA TCC ATG GCT CAG-3′)/16S rDNA-R (5′-AAG GAG GTG ATC CAG CC-3′)

dependent variables

Identification of isolates based on the 16S rDNA gene amplification

control variables

Bacterial genome extraction kit (Tiangen, China) following the manufacturer's protocols
Previously described method (24) for PCR

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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